While debugging the ASP.Net web application in Visual Studio 2005, it throws an error Unable to Start Debugging on the Web Server. This error occurs when Visual Studio 2005 tries to access the IIS 7 metabase and it fails to bind the IIS 7 virtual directory read/write permissions and IIS default port 80 to run the localhost ASP.Net web site. At that time Visual Studio 2005 fails to start the debugging mode for server end code of ASP.Net and throws the error Unable to Start Debugging on the Web Server.

Following are some steps that you can follow to configure your IIS 7 setting in Windows Vista or IIS 5 or later settings in Windows XP:

• Create the virtual directory of your web application that you are trying to debug.
  You can get the steps here to create virtual directory in Windows XP and create virtual directory in IIS 7 of Windows Vista.
  
  
• Apply the integrated windows authentication to the Default web site in IIS.
  You can follow the steps to apply integrated windows authentication in IIS

Learn how to install ASP.Net 2.0